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Saturday, April 13, 2013

Determining the Isotonic solution in Sheep Erythrocytes Isotonic solution of red blood cells (Laboratory) red blood cell sizes in saline red blood cell expansion in salt

Introduction

Every cell is selectively permeable to different molecules. This type of selectively is caused by a semi-permeable tissue layer, which al pocket-sizes the movement of certain molecules across it. water exchange tidy sum be measu violent in 2 ways: RBC osmotic permeableness is measured, and diffusional water permeability is measured (Benga and Borza 1995). Diffusion is the movement of high dumbness to low concentration. The diffusion of water across a permeable membrane is called osmosis. Water concentration in red pipeline cells can cause three different situations: hypertonic solution, isotonic solution, and a hypotonic solution. A hypertonic solution is when there is a higher concentration of table salts dissolved international the cell and unmixed water inside the cell, the water rushes out of the cell to get word to dilute the salt solution causing the cell to diminish up. An isotonic solution is where the water has achieved equilibrium with concentrations inside and outside of the cell, so there is no alteration of the cell. A hypotonic solution is where there is a higher concentration of salt dissolved inside the cell and a higher concentration of water outside the cell, so the water rushes into the cell onerous to dilute it causing swelling of the cell. If this takes place too rapidly, it will lead to the cell bursting.

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Knowing the isotonic solution of red blood cells is important in many ways much(prenominal) as filtration because the membranes flux causes the cell to become larger or smaller so if it is too large it can not be filtered through certain size capillaries (Tuvia 1992). Also, red blood cells can only operate in an isotonic state, in a hypotonic solution the cell bursts leaving no cell at all, but in a hypertonic solution the cell shrinks, which decreases the surface area and its viability to uptake oxygen...

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